human saa1 elisa kit  (R&D Systems)

Journal: Journal of Neuroinflammation

Article Title: Single-cell RNA sequencing uncovers neutrophil clusters associated with autoimmune neuroinflammation

doi: 10.1186/s12974-026-03772-9

Figure Lengend Snippet: Enhanced SAA1 expression and NET formation in plasma of MS patients. (A) SAA1 protein expression was quantified by ELISA in plasma samples collected from MS patients and HC. (B) H3.1-nucleosome expression was quantified by ELISA in plasma samples collected from MS patients and HC

Article Snippet: The human SAA1 ELISA kit (DY3019-05; R & D Systems, Minneapolis, MN) detected human SAA1 in plasma samples from MS patients and HC.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Journal of Advanced Research

Article Title: Characterization of immune features and discovery of potential biomarkers for ankylosing spondylitis using deep plasma proteomics

doi: 10.1016/j.jare.2025.05.052

Figure Lengend Snippet: Verification of potential biomarkers for AS. (A) Expression levels of SAA1, FERMT3, ILK, and TLN1 in plasma samples from active-stage AS patients, stable-stage AS patients, and healthy controls measured by proteomics. ROC curves for the individual protein (SAA1, FERMT3, ILK, and TLN1) as a predictor to classify AS patients from healthy controls (B), and active-stage AS patients from stable-stage AS patients (C). (D) ELISA analysis of SAA1, FERMT3, ILK, and TLN1 expressions in the plasma samples from an independent validation cohort of healthy controls (N, n = 24), active-stage AS (A, n = 27), and stable-stage AS patients (S, n = 28). Statistical analyses employed Wilcoxon rank-sum test for intergroup comparisons, with results reported as mean ± SEM. Significance thresholds based on BH-adjusted p -values were defined as: *, <0.05; **, <0.01; ***, <0.001.

Article Snippet: The human serum amyloid A (SAA1) ELISA kit (#OKIA00083) was purchased from Aviva Systems Biology Company (San Diego, CA, USA).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: Arthritis Research & Therapy

Article Title: Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis

doi: 10.1186/s13075-023-03243-z

Figure Lengend Snippet: Plasma concentration of SPP1, SAA1, and KNG1 in MDA5 + DM RP-ILD. A–C The concentrations of SPP1, SAA1, and KNG1 in the plasma from an independent cohort were determined by ELISA. P values were calculated by the ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. D–F ROC curve showed the AUC of the 3 verified proteins, respectively

Article Snippet: Following the manufacturer’s instructions, commercial ELISA kits were utilized for quantifying the levels of serum amyloid A1 (SAA1) (Boster Bio, China), V-Set And Immunoglobulin Domain Containing 4 (VSIG4), Coagulation Factor XI (F11) (both from CUSABIO, China), Coagulation Factor XIII A Chain (F13A1), Secreted phosphoprotein 1 (SPP1) and Kininogen 1 (KNG1) (all from Uscn life Science, China) in plasma samples from both patients and normal control subjects.

Techniques: Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Elevated Serum Amyloid A Levels Contribute to Increased Platelet Adhesion in COVID-19 Patients.

doi: 10.3390/ijms232214243

Figure Lengend Snippet: Figure 1. SAA is bound to platelets from COVID-19 patients. (A) Mass spectrometry was used to identify and quantify proteins overexpressed in purified COVID-19 platelets compared to controls. Shown are proteins whose levels were consistently elevated in two independent experiments. Values indicate the average fold-change (FC) in the relative label-free quantification intensity between the COVID-19 and the control samples (n = 4 in each group). Unpaired Student’s t-test was used to determine the significance. (B) Platelet lysates from control or COVID-19 donors were separated using SDS-PAGE. SAA was detected using a specific antibody. Tubulin was used as a loading control. Results are representative of six controls and six COVID-19 patients. Numbers represent fold intensity of control densitometric values normalized to tubulin. (C) SAA levels in platelet lysates from controls (n = 17) or COVID-19 patients (n = 15) were determined using ELISA. The dashed line in the violin plot is the median. The dotted lines show the interquartile range. Unpaired t-test, **** p < 0.001. (D) Platelet lysates from control or COVID-19 donors separated according to disease severity were analyzed using SDS-PAGE (n = 6 controls, 9 mild, 5 moderate, and 20 severe disease patients). SAA was detected using a specific antibody. The results were normalized to tubulin and to the amount of total protein. Ordinary one-way ANOVA, Dunnett’s multiple comparisons compared to control, ns = non-significant, * p < 0.05.

Article Snippet: An SAA-specific ELISA (SAA1 DuoSet ELISA Kit, R&D Systems, MN USA) was used to detect and quantify the SAA levels in the platelets of healthy and COVID-19 patients according to the manufacturer’s instructions.

Techniques: Mass Spectrometry, Control, SDS Page, Enzyme-linked Immunosorbent Assay